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| 30.08.2008 | 14:00 | Quick Boys II | - | HVV Hollandia II | 7:2 (2:0) | ||
| 14:30 | HHC Hardenberg | - | RKSV Schijndel | 2:1 (0:1) | |||
| 14:30 | GVVV | - | SV Deurne | 4:2 i.E. | |||
| 14:30 | SHO Oud Beijerland | - | VV Papendrecht | 1:2 | |||
| 14:30 | VV Hoogeveen | - | Be Quick '28 | 1:3 | |||
| 14:30 | Rijnsburgse Boys | - | DESK Kaatsheuvel | 5:0 | |||
| 14:30 | Eems Boys | - | EHC Hoensbroek | 0:3 | |||
| 15:00 | IJsselmeervogels | - | VV Glanerbrug | 4:0 | |||
| 15:00 | Sparta Nijkerk | - | RKVVO Veldhoven | 4:0 | |||
| 16:00 | RKSV Groene Ster | - | FC Lisse | 1:2 n.V. | |||
| 17:00 | SV Argon | - | VV Capelle | 1:3 | |||
| 17:00 | VV De Bataven | - | ONS Sneek | 4:2 i.E. | |||
| 17:00 | SMVC Fair Play | - | AFC Ajax Zaterdag | 0:2 | |||
| 18:00 | VV UNA/Brinvast | - | Kozakken Boys | 0:2 | |||
| 18:00 | HSC'21 | - | DOTO Pernis | 2:1 | |||
| 18:00 | De Treffers | - | Excelsior '31 | 4:0 | |||
| 18:00 | VV Baronie | - | Quick Boys | 0:4 | |||
| 18:00 | Achilles'29 | - | Achilles Veen | 3:1 (2:0) | |||
| 18:00 | SV Spakenburg | - | VV Gemert | 9:8 i.E. | |||
| 31.08.2008 | 14:00 | RKVV Westlandia | - | HVV Hollandia | 4:5 i.E. | ||
| 14:00 | Xerxes DZB | - | Be Quick 1887 | 1:6 | |||
| 14:00 | WKE Emmen | - | Unitas '30 | 3:1 | |||
| 14:00 | DWV Amsterdam | - | FC Lienden | 0:1 | |||
| 14:00 | ASVB Blijham | - | VV Haaglandia | 0:10 |
