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| 09.09.2016 | 15:00 | MFK Vítkovice | - | FK Varnsdorf | 2:3 (1:1) | |
| 18:00 | Dynamo České Budějovice | - | FK Ústí nad Labem | 2:1 (1:0) | ||
| 10.09.2016 | 10:15 | FK Pardubice | - | SFC Opava | 0:2 (0:0) | |
| 10:15 | FK Fotbal Třinec | - | Sigma Olomouc | 1:2 (0:2) | ||
| 16:30 | FC Sellier & Bellot Vlašim | - | Baník Ostrava | 1:1 (1:1) | ||
| 17:00 | FK MAS Táborsko | - | MFK Frýdek-Místek, a.s. | 2:2 (2:1) | ||
| 17:00 | 1. SK Prostějov | - | 1. SC Znojmo | 0:2 (0:1) | ||
| 11.09.2016 | 10:15 | Viktoria Žižkov | - | FK Baník Sokolov | 2:2 (0:2) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Baník Ostrava | 6 | 4 | 2 | 0 | 12:4 | 8 | 14 |
| 2 |
|
Dynamo České Budějovice | 6 | 4 | 2 | 0 | 7:2 | 5 | 14 |
| 3 |
|
Sigma Olomouc | 6 | 3 | 3 | 0 | 8:4 | 4 | 12 |
| 4 |
|
SFC Opava | 6 | 3 | 2 | 1 | 11:7 | 4 | 11 |
| 5 |
|
FK Varnsdorf | 6 | 3 | 2 | 1 | 7:3 | 4 | 11 |
| 6 |
|
1. SC Znojmo | 6 | 3 | 1 | 2 | 13:9 | 4 | 10 |
| 7 |
|
MFK Frýdek-Místek, a.s. | 6 | 2 | 2 | 2 | 10:11 | -1 | 8 |
| 8 |
|
FK MAS Táborsko | 6 | 2 | 2 | 2 | 7:8 | -1 | 8 |
| 9 |
|
Viktoria Žižkov | 6 | 1 | 4 | 1 | 11:9 | 2 | 7 |
| 10 |
|
FK Baník Sokolov | 6 | 1 | 4 | 1 | 7:9 | -2 | 7 |
| 11 |
|
MFK Vítkovice | 6 | 2 | 0 | 4 | 6:10 | -4 | 6 |
| 12 |
|
FC Sellier & Bellot Vlašim | 6 | 1 | 2 | 3 | 10:12 | -2 | 5 |
| 13 |
|
FK Pardubice | 6 | 1 | 2 | 3 | 6:13 | -7 | 5 |
| 14 |
|
FK Fotbal Třinec | 6 | 1 | 1 | 4 | 10:14 | -4 | 4 |
| 15 |
|
FK Ústí nad Labem | 6 | 1 | 1 | 4 | 8:13 | -5 | 4 |
| 16 |
|
1. SK Prostějov | 6 | 0 | 2 | 4 | 4:9 | -5 | 2 |
| Aufstieg | |
| Abstieg |
