20.11.1954 Barnet FC - Southampton FC 1:4
Barrow AFC - Darlington FC 1:1
Bristol City - Southend United 1:2
Dorchester Town - Bedford Town 2:0
Reading FC - Colchester United 3:3
Walsall FC - Shrewsbury Town 5:2
Gillingham FC - Newport County 2:0
Grimsby Town - Halifax Town 2:1
Swindon Town - Crystal Palace 0:2
Bishop Auckland FC - Kettering Town 5:1
Tranmere Rovers - Rochdale AFC 3:3
Stockport County - Carlisle United 0:1
Queens Park Rangers - Walthamstow Ave 2:2
Accrington Stanley - Creswell Colliery 7:1
Barnsley FC - Wigan Athletic 3:2
Brentford FC - Nuneaton Borough FC 2:1
Crook Town AFC - Stanley United FC 5:3
Northampton Town - Coventry City 0:1
Brighton & Hove Albion - Tunbridge Wells 5:0
Norwich City - Headington United 4:2
Bradford City - Mansfield Town 3:1
Millwall FC - Exeter City 3:2
Oldham Athletic - Crewe Alexandra 1:0
Frome Town FC - Leyton Orient 0:3
Bradford Park Avenue - Southport FC 2:0
Hartlepools United - Chesterfield FC 1:0
Selby Town - Rhyl FC 2:1
Torquay United - Cambridge United 4:0
Workington AFC - Hyde United 5:1
York City - Scarborough FC 3:2
Netherfield - Wrexham AFC 3:3
Aldershot FC - Chelmsford City 3:1
Horden Colliery Welfare ACF - Scunthorpe & Lindsey 0:1
Gateshead FC - Chester City 6:0
Hounslow - Hastings United 2:4
Boston United - Blyth Spartans 1:1
Merthyr Tydfil FC - Wellington Town 1:1
Hinckley United - Newport IOW 4:3
Barnstaple Town - Bournemouth and Boscombe Athletic 1:4
Corby Town - Watford FC 0:2
Entscheidungsspiele
23.11.1954 Rochdale AFC - Tranmere Rovers 1:0
24.11.1954 Darlington FC - Barrow AFC 2:1
Wrexham AFC - Netherfield 4:0
Blyth Spartans - Boston United 5:4
Wellington Town - Merthyr Tydfil FC 1:6
25.11.1954 Colchester United - Reading FC 1:2
Walthamstow Ave - Queens Park Rangers 2:2
Queens Park Rangers - Walthamstow Ave 0:4